Re: your mail

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From:"M. Brown" <marianb@u.washington.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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The same for me, I would cut slides, put in a slide box at -80 with a
small amt.of desiccant wrapped in gauze. I didn't fix the slides.Had no
problems even after 1yr.
Marianne




On Fri, 5 Mar 1999, Ian Montgomery wrote:

> >Date: Thu, 04 Mar 1999 14:57:56 -0500
> >From: "Patterson, Noelle" <PattersonN@nmripo.nmri.nnmc.navy.mil>
> >To: Histonet <HistoNet@pathology.swmed.edu>
> >MIME-version: 1.0
> >
> >Hi!  I am collecting cryostat section storage methods.  Please include
> >whether you fix sections before storage and what temp. you store slides at.
> >Do you use a dessicant or not?  Once stored, how do you prepare them for
> >use/stainings.  Does your storage method permit later routine, special,
> >and/or immunohistochemical stains with the same quality as same day cut and
> >stained (not stored) specimens?
> >
> >Thanks,
> >Noelle Patterson
> >Naval Medical Research Center
> >Bethesda, Md
> >pattersonn@NMRIPO.NMRI.NNMC.NAVY.MIL
> >
>
> Noelle,
> 	Have only ever stored for histochemistry. Store at -80 in a sealed
> box with some silca gel. Remove sections as necessary, bring back to
> ambient and stain. Although ATPase works I find that it is better on fresh
> sections. Human material is more forgiving in this respect than other
> species.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>




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