Tami,


What you are describing sounds like poorly processed tissue. The problem
may be the tissue processor.  Which processor do you have?  Usually, uterus
is fixed in a couple of days.

Rande Kline HT (ASCP)
Technical Services
EM Science





Tami Lee <Tami.Lee@css.unc.edu> on 03/08/99 08:50:40 AM

To:   HistoNet@pathology.swmed.edu
cc:
Subject:  troublesome tissues




Hi everyone,
     I am trying to fix, process, and section breast and uterine tissue.
Although the uterus was fixed
in 10% NBF for several days, it still appeared to have areas that were
poorly
fixed.  The processing
schedules that I have been using are 12 hours long, and do not seem to be
effective, especially
on the breast tissue.  Does anyone have any advice they wouldn't mind
sharing?
I have not been able
to find anything in our laboratory's textbooks.
Thanks in advance,
Tami Lee