Re: troublesome tissues

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To:Tami Lee <>
Date:Mon, 08 Mar 1999 10:57:35 -0500
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What you are describing sounds like poorly processed tissue. The problem
may be the tissue processor.  Which processor do you have?  Usually, uterus
is fixed in a couple of days.

Rande Kline HT (ASCP)
Technical Services
EM Science

Tami Lee <> on 03/08/99 08:50:40 AM

Subject:  troublesome tissues

Hi everyone,
     I am trying to fix, process, and section breast and uterine tissue.
Although the uterus was fixed
in 10% NBF for several days, it still appeared to have areas that were
fixed.  The processing
schedules that I have been using are 12 hours long, and do not seem to be
effective, especially
on the breast tissue.  Does anyone have any advice they wouldn't mind
I have not been able
to find anything in our laboratory's textbooks.
Thanks in advance,
Tami Lee

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