Re: tissue processing

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From:Tim Morken <>
Date:Thu, 11 Mar 1999 10:48:34 -0500 (EST)


I guess the question to ask is, are the urgent samples urgent enough to 
delay other samples? 

You can always reduce the processing time, but there will be trade offs, 
as you allude to with your coments about size of samples. Small samples 
can be processed very quickly (ie biospy samples are often done in a 
couple hours, but are only 1 mm thick). 

I am assuming you have only one processor. If that is the case you can 
suggest running urgent samples alone on a rush scedule and delaying 
other samples for a normal run. 

The point to make is that in no case should the integrity of any sample 
be degraded by the need to rush certain samples.

Tim Morken, B.A., EMT(MSA), HTL(ASCP) 
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


FAX:  (404)639-3043

----Original Message Follows----
From: Deborah Faichney <>
To: 'histonet' <>
Subject: tissue processing
Date: Thu, 11 Mar 1999 12:46:13 +0000

Hi ,

We work with fish tissues, mainly Salmonids.  Our pathologist has asked 
we can reduce the overnight processing to 16 hours from 21 hours, thus,
enabling him to announce urgent samples late afternoon!!

 We use a series of Industrial Methylated spirit, Ethanol, Chloroform 
Paraffin.  Our tissue samples can be quite large (up to 3cmx1cm), 
they are below 5mmx5mm.

Any ideas or comments will be gratefully received.

Debbie Faichney
Institute of Aquaculture
Stirling University

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