Re: silver staining endothelium

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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
To:HistoNet@Pathology.swmed.edu
Reply-To:
Date:Fri, 12 Mar 1999 14:56:46 +0000
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>X-Sender: ib@jeack.com.au
>Mime-Version: 1.0
>Date: Fri, 12 Mar 1999 19:28:45 +1000
>To: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
>From: Ian <ib@jeack.com.au>
>Subject: Re: silver staining endothelium
>
>Thanks Ian (great name!)for the reference. I have mislayed my old Carelton's
>text but I should be able to find one. Do you have any experience with the
>technique? I am trying to demonstrate monolayer formation by seeded human
>and sheep endothelial cells onto different biosynthetic surfaces. I am
>forced to use an ICC method on en face preparations because the underlying
>matrix has a cellular component. A nice silver stain would certainly help
>with my interpretation of the preparations. If you have any hints I would
>certainly appreciate any help. Thanks, Ian Birchall.
>
Ian,
	Just looked up Carleton, never tried it myself, although would like
to hear from anyone who has. If you can't find a copy let me know and I'll
fax the technique, but, don't tell anybody, I never wrote this sentence.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk





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