Re: p53 staining in cryosections

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From:"Technical Services" <> (by way of histonet)
To:histonet <>
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Hi Pat,

Our p53 antibody works in frozen sections, but we do recommend a lesser
dilution than we do for FFPE tissue (it's about 1/2 as dilute for frozens,
or for FFPE tissue it is twice as dilute). Fixing with acetone should
certainly be adequate, but you could also use acetone/methanol, or

Joel Weisenberger
DAKO Corporation
Technical Services
800-235-5743 x5325

-----Original Message-----
From: Pat Eden <>
To: 'HistoNet Server' <>
Date: Wednesday, March 03, 1999 9:27 AM
Subject: p53 staining in cryosections

>I am looking for tips on immunohistochemical staining of p53 in frozen
>sections of colon tumors.  I am working with an antibody (Sigma
>BP53.12)which works well with paraffin sections, however, it doesn't seem
>to be as effective with frozen sections.  I am fixing the tissue in cold
>acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB
>detection system.  Any advice on immunohistochemical protocols on frozen
>sections or p53 antibodies would be greatly appreciated.

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