Hi Pat,

Our p53 antibody works in frozen sections, but we do recommend a lesser
dilution than we do for FFPE tissue (it's about 1/2 as dilute for frozens,
or for FFPE tissue it is twice as dilute). Fixing with acetone should
certainly be adequate, but you could also use acetone/methanol, or
acetone/methanol/formalin.

Joel Weisenberger
DAKO Corporation
Technical Services
800-235-5743 x5325
techserv@dakousa.com

-----Original Message-----
From: Pat Eden <pat.eden@spectrum-ferguson.org>
To: 'HistoNet Server' <HistoNet@Pathology.swmed.edu>
Date: Wednesday, March 03, 1999 9:27 AM
Subject: p53 staining in cryosections


>I am looking for tips on immunohistochemical staining of p53 in frozen
>sections of colon tumors.  I am working with an antibody (Sigma
>BP53.12)which works well with paraffin sections, however, it doesn't seem
>to be as effective with frozen sections.  I am fixing the tissue in cold
>acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB
>detection system.  Any advice on immunohistochemical protocols on frozen
>sections or p53 antibodies would be greatly appreciated.
>