Re: p53 staining in cryosections

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From:"M. Brown" <> (by way of histonet)
To:histonet <>
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I used to stain FS sections by just air drying slide for at least 30min,
then go into PBS and proceed. Biocare Medical's p53 works great on acetone
fixed fs slides. 800-799-9499
Marianne Brown

On Wed, 3 Mar 1999, Pat Eden wrote:

> I am looking for tips on immunohistochemical staining of p53 in frozen
> sections of colon tumors.  I am working with an antibody (Sigma
> BP53.12)which works well with paraffin sections, however, it doesn't seem
> to be as effective with frozen sections.  I am fixing the tissue in cold
> acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB
> detection system.  Any advice on immunohistochemical protocols on frozen
> sections or p53 antibodies would be greatly appreciated.

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