Ian,

I agree with your comments completely.   I would also add that if I wanted to
look at 1-2 micron sections paraffin would NOT be my embedding media of choice.
 I would go with one of the plastics,  much less distortion.

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**

-- Begin original message --

> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Date: Thu, 04 Mar 1999 13:53:19 +0000
> Subject: =?iso-8859-1?Q?1=B5m?= sections.
> To: HistoNet@pathology.swmed.edu
>
>       Perhaps the Pathologists can answer my question. Why do you need a
> 1µm wax section. I was trained to use the fine focus control on a
> microscope and my brain to think 3 dimensionally, after all we live in a 3
> dimensional world. Moving the fine focus control up and down reveals the
> wonders and delights of a specimen. Structures that a 1µm section will
> never demonstrate become apparant. Cell and tissue relationships flood your
> eyes and brain with their striking beauty, so why hide them with 1µm
> sections.
>       I accept reasonably thin sections are necessary  for ICC, but why
> 1µm.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>

-- End original message --