Re: frozen section staining

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From:Donna Carr <dkc@odsgc.net> (by way of histonet)
To:histonet <histonet@magicnet.net>
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We use 1 minute in Pen-Fix
        rinse in tap water
        30 secs in Hematoxilyn
        rinse in tap water X2
        blue in ammonia water 7 dips
        12 dips in tap water
        12 dips in 95% ROH
        3 dips in Eosin
        12 dips in following:
            95% X 2
            100% X 3
            clearing medium X 3
That's it.




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