Re: eosin staining

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From:"R.Wadley" <>
Date:Tue, 09 Mar 1999 09:56:03 +1000
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	Dear Gino,

	I add acetic acid when I prepare the stain, in a lab situation I prefer
the running of a control slide first thing in the morning.  Eosin (&
haematoxylin) should be filtered every morning.  Depending on the number of
slides you do each day eosin can be changed weekly.  This is why you do the
control slide, if the eosin looks a bit off by Thursday or Friday morning
then change it then, don't wait to Monday morning!
	I once worked in a lab (in a major capitol city hospital) that did not run
control H&E slides, a whole batch of patient slides were sent to the
pathologists without any eosin staining, because one of the tech's had
forgotten to load eosin into the autostainer.  Actually checking the
control slide (& at least a representative slide from each batch) is as
important as actually staining the thing in the first place!
	My preference is 1% eosin Y in 70% ethanol with 0.05% acetic acid.  This
stain does not leach out excessively during washing or dehydration, &
provides vivid cytoplasmic staining for photography purposes.  The volume I
prepare at any one time depends on the number of slides processed per day.


	Rob W.

At 10:47 AM 3/8/99 -0500, you wrote:
>can you please share your QC procedures for eosin staining in H&E
>slides. Do you add acetic acid to eosin prior to staining? do you run
>control slides? if so, how often during the day or week. Do you filter
>fresh eosin daily or filter and reuse? 

R. Wadley, B.App.Sc, M.L.S
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591
	(Under development)

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