Re: aggressive antigen retrieval

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From:Janet Maass <> (by way of histonet)
To:histonet <>
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For formic acid treatment try air drying slides at room temp overnight,
then the next day dry in a 70 oven for 30 minutes.  I have found the
adhesive coated slides aminosilane from Newcomer Supply has worked better
than plus slides from Erie.  I can not explain why.  Newcomer Supply 2217 B
Parview Rd. Middleton, WI 53562
800-383-7799, 608-831-7888, fax 608-831-0866

Janet Maass

> From: Joyce Kotzuk <>
> To:
> Subject: aggressive antigen retrieval
> Date: Friday, March 05, 1999 8:38 AM
> I have problems with section adherence when I try aggressive antigen
retrieval methods such as multiple microwavings, formic acid treatments
etc. I am currently using Vectabond treated slides; I bake the sections at
37 degrees overnight right after cutting, then store at room temp. till I
need them. they go in a 60 degree oven for 30 minutes just prior to
deparaffinization in xylene. Any suggestions as to how to keep sections on
the slides when using severe antigen retrieval methods would be greatly
appreciated. Thanks.
> Joyce Kotzuk
> Univ. of New Mexico pathology

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