Joice, are you putting Gelatin in the water bath? You can't do
that.Biocare Medical 800 799 9499 has excellent charged slides very
reasonable in price. Marianne




On Fri, 5 Mar 1999, sonja digiacomo wrote:

> I have been off work for one week and expect to be off one more week but
> when I return I will send you and brand of plus slides which are used in
> our lab.  I have not had any problem loosing secions and I do all the
> immuno and even have pressure cooked slides in citric acid buffer in the
> microwave for 17 minutes.  S.DiGiacomo,Prostate Diagnositc Laboratory,
> Denver, Colo.
>
>
> >From histonet@pathology.swmed.edu Fri Mar  5 08:09:05 1999
> >Received: from 129.112.18.39 ([129.112.18.39])
> > by SWAX32.SWMED.EDU (PMDF V5.1-8 #18228)
> > with SMTP id <01J8GU5C5NTWA782W0@SWAX32.SWMED.EDU>; Fri,
> > 5 Mar 1999 10:08:45 CST
> >Date: Fri, 05 Mar 1999 08:38:41 -0700
> >From: Joyce Kotzuk <JKotzuk@salud.unm.edu>
> >Subject: aggressive antigen retrieval
> >To: HistoNet@Pathology.swmed.edu
> >Message-id: <s6df9821.014@salud>
> >MIME-version: 1.0
> >Content-type: text/plain; charset=US-ASCII
> >Content-transfer-encoding: quoted-printable
> >
> >I have problems with section adherence when I try aggressive antigen =
> >retrieval methods such as multiple microwavings, formic acid treatments
> =
> >etc. I am currently using Vectabond treated slides; I bake the sections
> at =
> >37 degrees overnight right after cutting, then store at room temp. till
> I =
> >need them. they go in a 60 degree oven for 30 minutes just prior to =
> >deparaffinization in xylene. Any suggestions as to how to keep sections
> on =
> >the slides when using severe antigen retrieval methods would be greatly
> =
> >appreciated. Thanks.
> >Joyce Kotzuk
> >Univ. of New Mexico pathology
> >
> >
> >
>
>
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