Re: TMJ disc processing/JB-4 media

<< Previous Message | Next Message >> ("P. Emry"),
Date:Wed, 10 Mar 1999 11:41:17 -0500 (Eastern Standard Time)

Yes it is miscible with water.  I would use a graded series of monomer and
water, probably starting at 30% monomer/70% water and work my way up to 100%
monomer.  I used to do this with tissues years ago.

-- Begin original message --

> Hi Netters,
> I have a grad student that will be looking at tmj discs.  She has not
> found a protocol for this so we would appreciate some advice on the
> procedure.  She had heard that frozen sections might be best since they
> have so much water in them.
> I got the catalog from polysciences with JB-4 media advertised.  If it is
> miscible with water would that make it a good media to use for the discs?
> Any and all experience/advice with discs and  JB-4 would be welcomed.
> Trisha Emry
> Orthodontices Research Lab
> U of Washington, Seattle

-- End original message --

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**

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