Ian,

We use Dako's antibody for smooth muscle actin.  It works quite nicely on
human and rat, I would assume that it will work on mice but we haven't
tryed that yet.  We use a 1:100 dilution of the primary 10 min. at room
temp. and we use Dako's Envision kit secondary is also 10 min. at RT. 
Works like a charm and it's quick and simple.

-- Begin original message --

>
> 
>       Anyone using an good reliable antibody for smooth muscle cells.
> Will be staining human and possibly mouse tissue.
> Ian.
> 
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk

-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone 
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress
...
But I repeat myself.-Mark Twain**