Re: Nuclear Bubbling Revisited

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To:"Gamble,Marilyn S" <>
Date:Mon, 15 Mar 1999 09:21:59 -0500
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I don't believe recycled xylene is the problem.  I always used it.

But,, you may want to send a sample out to the manufacturer of the recycler
and have them analyze it.  I  would do this periodically,  Sometimes the
program has to be adjusted.

Also, for biopsies I always had a shorter processing program.  I was
fortunate to have a Shandon processor with four processing modules.

Rande Kline HT (ASCP)
Technical Services
EM Science

"Gamble,Marilyn S" <> on 03/12/99 06:08:08 PM

To:   'Histonet' <>
Subject:  Nuclear Bubbling Revisited

Nuclear bubbling continues to be a problem with our prostate needle
biopsies.  Prior consensus of the Histonetters was overwhelmingly -
Excessive Heat.  We adjusted the drying oven for slides, so excessive heat
shouldn't be our problem.  Paraffin temperatures are the norm.   I heard
through the (pathologist) grapevine, that someone said recycled xylene can
cause this.   Can this be so?   (We do used recycled xylene).   How/Why?
there a solution?
Any other ideas?.......And why so selective with pnb's ?  Is this a male

Marilyn (Perplexed in California!)

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