Dear Ellen,
I passed the step of looking for a suitable antibody for paraffin mouse
sections. I would recommend rat anti-human CD3 (monoclonal) from Serotec.
You need to cook your slides in microwave 2 x 5 min in citrate buffer. Dil.
of primary antibody 1:50 (PAP method).
I tried also antibody from Dako but I had a big problem with background.
Good luck,
Grazyna





Ellen M McAvoy <mcavoye@bms.com> on 09.03.99 15:19:16

To:   histonet@Pathology.swmed.edu
cc:    (bcc: Grazyna Wieczorek/PH/Novartis)
Subject:  Mouse T-cells




I am looking for a T-cell reagent for use on paraffin mouse
tissues: spleen and lymph node.  Anyone have any suggestions?