Re: Irrelevant antiibodies versus omiting the primary Ab for IHC control,
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From: | Jeff Silverman <peptolab@hamptons.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Tony and Netters,
My sentiments exactly, that's one reason to always run a panel and not
single antibodies, this way we at least can derive some useful information
from our negative controls.
Jeff SIlverman
----------
> From: Tony Henwood <henwood@mail.one.net.au>
> To: HistoNet@Pathology.swmed.edu; Bruce A Rasmussen
<brasmuss@osf1.gmu.edu>
> Subject: Re: omiting the primary Ab for IHC control,
> Date: Tuesday, March 02, 1999 5:48 PM
>
> It is a good idea to include a negative control with each case. Often
> when a panel of antibodies (eg S100, keratin and LCAg) are used the
> "negative" control is superfluous since one hopes that the tumour
> will be negative for one of the panel antibodies used.
> Tony Henwood
> www2.one.net.au/~henwood
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