We use a Tris-saline wash buffer with Tween 20 (no serum) that is 
recommended by DAKO for their autostainer (which we use). It has a Tween 
20 concentration of 0.0005% (2 ml in 4L).

I don't know the rationale of using acetylated BSA so can't  comment on 
that.

Tim Morken, B.A., EMT(MSA), HTL(ASCP) 
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

FAX:  (404)639-3043


----Original Message Follows----
From: rschoonh@sph.unc.edu
To: Patsy.Ruegg@UCHSC.edu, histonet@pathology.swmed.edu
Subject: Re: IHC Question
Date: Thu, 11 Mar 1999 08:30:55 -0500 (Eastern Standard Time)

Patsy,

I routinely add Tween 20 to my wash buffer but not BSA.  I do use 1% BSA 
in my
antibody dilution buffers but not Tween 20.  I have also noticed that
concentrations of Tween 20 noted in the literature varies from 0.05% to 
1.0%, I
have always used 1.0% with no problems but I'm interseted in what others 
are
using and why.

-- Begin original message --
> 
> I have been having trouble with non-specific background staining and 
have
> gone to putting acetylated BSA in my wash buffer, which helps.  My 
question
> is:  is there any reason not to also put the TWEEN in the wash buffer, 
it
> seems that I read something a while back suggesting that they should 
not be
> used together??????
> Patsy Ruegg
> 
> 

-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone 
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

**Suppose you were an idiot... And suppose you were a member of Congress 
...
But I repeat myself.-Mark Twain**












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