I have always put Tween 20 in my buffers. I have never had to add BSA to
the wash buffer. After I made sure both Primary and secondary antibodies
were optimally diluted, if I still had background staining, I did a
blocking with 3%BSA + Tween 20 after the link antibody. This seemed to
work well.
Marianne




On Wed, 10 Mar 1999 Patsy.Ruegg@UCHSC.edu wrote:

> I have been having trouble with non-specific background staining and have
> gone to putting acetylated BSA in my wash buffer, which helps.  My question
> is:  is there any reason not to also put the TWEEN in the wash buffer, it
> seems that I read something a while back suggesting that they should not be
> used together??????
> Patsy Ruegg
> 
>