Re: Daily Digest

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From:Edward Henry <morningstar_group@yahoo.com>
To:HistoNet Server <HistoNet@Pathology.swmed.edu>
Reply-To:
Date:Mon, 08 Mar 1999 04:55:54 -0800 (PST)
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---HistoNet Server <HistoNet@Pathology.swmed.edu> wrote:
>
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 01:31:15 -0600
> From: Sharyn S Caplan <phinny1@juno.com>
> Subject: Dr. Elias
> 
> Can anyone please how to get in touch with Jules Elias?    I have an
> immuno question for him
> 
> Thanks 
> Sharyn Caplan  HT
> Orlando, Florida
> 
> Sharyn1678@yahoo.com
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at
http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 03:15:49 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Sirius Red.
> 
> 	This has been asked before, I took details of the technique and
> used it very successfully.  But, guess who, foolishly, didn't enter
it into
> his techniques book. Please, can the method for Sirius Red be posted
again.
> The reference would also be nice.
> Ian.
> 
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 03:16:15 -0600
> From: DORIT ZHARHARY <d_zharhary@sigma.co.il>
> Subject: RE: p53 staining in cryosections
> 
> The fixation method recommended for frozen sections using this
antibody is:
> 1. Methanol / Acetone (1:1 v/v), pre cooled at -20oC, 10 minutes,
followed by
> PBS wash.
> 2. Try prolonging the incubation time with the antibody, even
overnight at
> 4oC, and try several antibody dilutions.
> 
> Dorit Zharhary
> 
> 
> - ----------
> From:  Pat Eden
> Sent:  eai oaeoe 03 ioo 1999 18:55
> To:  'HistoNet Server'
> Subject:  p53 staining in cryosections
> 
> I am looking for tips on immunohistochemical staining of p53 in
frozen 
> sections of colon tumors.  I am working with an antibody (Sigma 
> BP53.12)which works well with paraffin sections, however, it doesn't
seem 
> to be as effective with frozen sections.  I am fixing the tissue in
cold 
> acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase DAB 
> detection system.  Any advice on immunohistochemical protocols on
frozen 
> sections or p53 antibodies would be greatly appreciated.  
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 05:16:13 -0600
> From: Anne van Binsbergen <anneb@mail.saimr.wits.ac.za>
> Subject: bmt decal
> 
> Hi there Histonetters,
> 
> Our recipe for EDTA decal sol for BMT is as follows:
> 
> 1750ml dist H20
> 250g EDTA (disodium salt)
> 
> Mix together and neutralise with NH4OH (use litmus paper to check) 
> (NH+  act as chelating agents too)
> 
> After this the solution turns cloudy - this is cleared by continuously
> stirring for about 30mins
> 
> 
> 
> We decal overnight (after checking for proper fixation - usually
24hrs) and
> check manually  - sometimes the tissue is left in the decal fluid
for a
> further 24hrs - specimens containing cortical bone will take much
longer.
> 
> We put the decal fluid into the specimen 'pot' - about 50 times the
volume
> of the tissue
> 
> Don't use any metal cassette lids
> 
> The tissue does not need to be washed after decal and before
processing
> 
> Gloves are not necessary as the decal solution is not toxic or
corrosive
> 
> Our results speak for themselves!!
> 
> happy decalling
> 
> Annie (in Africa)
> Anne S. van Binsbergen
> T.I.C. Anatomical Pathology
> S.A.I.M.R. 
> Chris Hani Baragwanath Hospital
> PO Box 1038
> Johannesburg 2000
> South Africa
> 
> tel: 011 4898711
> cell: 083 4403343
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 06:46:04 -0600
> From: "Rae Staskiewicz" <raestask@galesburg.net>
> Subject: Glenda Hood's Address
> 
> Could someone send me the e-mail address of Glenda Hood?
> Thanks all!
> 
> Rae Ann Staskiewicz
> Galesburg Animal Disease Lab
> Galesburg, IL
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 08:01:13 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: 1um sections.
> 
> 	Perhaps the Pathologists can answer my question. Why do you need a
> 1um wax section. I was trained to use the fine focus control on a
> microscope and my brain to think 3 dimensionally, after all we live
in a 3
> dimensional world. Moving the fine focus control up and down reveals
the
> wonders and delights of a specimen. Structures that a 1um section will
> never demonstrate become apparant. Cell and tissue relationships
flood your
> eyes and brain with their striking beauty, so why hide them with 1um
> sections.
> 	I accept reasonably thin sections are necessary  for ICC, but why
> 1um.
> Ian.
> 
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 4 Mar 1999 10:00:37 -0600
> From: "M. Brown" <marianb@u.washington.edu>
> Subject: Re: p53 staining in cryosections
> 
> I used to stain FS sections by just air drying slide for at least
30min,
> then go into PBS and proceed. Biocare Medical's p53 works great on
acetone
> fixed fs slides. 800-799-9499
> Marianne Brown
> 
> 
> 
> 
> On Wed, 3 Mar 1999, Pat Eden wrote:
> 
> > I am looking for tips on immunohistochemical staining of p53 in
frozen 
> > sections of colon tumors.  I am working with an antibody (Sigma 
> > BP53.12)which works well with paraffin sections, however, it
doesn't seem 
> > to be as effective with frozen sections.  I am fixing the tissue
in cold 
> > acetone for ten minutes, and using a Sigma ExtrAvidin peroxidase
DAB 
> > detection system.  Any advice on immunohistochemical protocols on
frozen 
> > sections or p53 antibodies would be greatly appreciated.  
> > 
> 
=== message truncated ===

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