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From:Tim Morken <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

Here is a lot of info, including good web sites, about CJD precautions
and disinfection. The CAP web site has good disinfection procedures.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333


FAX:  (404)639-3043

Journal article:
Handling Creutzveldt-Jakcob disease Tissues in the Histology Laboratory,
Titford, M. and Bastian, F.O., J Histotech, V12, No.3 Sept 1989

If you have access to the internet you can go to these sites:

Search Altavista for "CJD" at
there are many articles concerning CJD and V-CJD from Britain.

CAP: Detailed information on handling CJD in the autopsy suite and the
lab, with references.

For all CDC information on CJD, go to to the main CDC web page and
search for "CJD" :

CJD Epidemiology, risk factors and decontamination:

Media facts:

Udatated  info at (reproduced below)

AGENT: Transmissible Spongiform Encephalopathies (Creutzfeldt-Jakob,
     and related agents)

     Laboratory-associated infections with the transmissible spongiform
encephalopathies (prion
     diseases) have not been documented. However, there is evidence that
     disease (CJD) has been transmitted iatrogenically to patients by
corneal transplants, dura
     mater grafts and growth hormone extracted from human pituitary
glands, and by exposure
     to contaminated electroencephalographic electrodes (99). Infection
is always fatal. There is
     no known nonhuman reservoir for CJD or kuru. Nonhuman primates and
other laboratory
     animals have been infected by inoculation, but there is no evidence
of secondary
     transmission. Scrapie of sheep and goats, bovine spongiform
encephalopathy and mink
     encephalopathy are transmissible spongiform encephalopathies of
animals that are similar to
     the human transmissible diseases. However, there is no evidence
that the animal diseases
     can be transmitted to man.

     LABORATORY HAZARDS: High titers of a transmissible agent have been
demonstrated in
     the brain and spinal cord of persons with kuru. In persons with
Creutzfeldt-Jakob disease
     and its Gerstmann-Straussler-Schenker Syndrome variants, a similar
transmissible agent has
     been demonstrated in the brain, spleen, liver, lymph nodes, lungs,
spinal cord, kidneys,
     cornea and lens, and in spinal fluid and blood. Accidental
parenteral inoculation, especially
     of nerve tissues, including formalin-fixed specimens, is extremely
hazardous. Although
     non-nerve tissues are less often infectious, all tissues of humans
and animals infected with
     these agents should be considered potentially hazardous. The risk
of infection from aerosols,
     droplets, and exposure to intact skin, gastric and mucous membranes
is not known;
     however, there is no evidence of contact or aerosol transmission.
These agents are
     characterized by extreme resistance to conventional inactivation
procedures including
     irradiation, boiling, dry heat and chemicals (formalin,
betapropiolactone, alcohols);
     however, they are inactivated by 1 N NaOH, sodium hypochlorite (>2%
free chlorine
     concentration) and steam autoclaving at 134 degrees C for 1 hour.

     RECOMMENDED PRECAUTIONS: Biosafety Level 2 practices and facilities
are recommended
     for all activities utilizing known or potentially infectious
tissues and fluids from
     naturally-infected humans and from experimentally infected animals.
Extreme care must be
     taken to avoid accidental autoinoculation, or other traumatic
parenteral inoculations of
     infectious tissues and fluids (76). Although there is no evidence
to suggest that aerosol
     transmission occurs in the natural disease, it is prudent to avoid
the generation of aerosols
     or droplets during the manipulation of tissues or fluids, and
during the necropsy of
     experimental animals. It is further strongly recommended that
gloves be worn for activities
     that provide the opportunity for skin contact with infectious
tissues and fluids.
     Formaldehyde-fixed and paraffin-embedded tissues, especially of the
brain, remain
     infectious. It is recommended that formalin-fixed tissues from
suspected cases of
     transmissible encephalopathy be immersed in 96% formic acid for 30
minutes before
     histopathologic processing (15). Vaccines are not available for use
in humans (51).

----Original Message Follows----
Date: Wed, 03 Mar 1999 09:24:22 -0500
From: "Celebre, Gino" <celebre@HAMCIVHOS.ON.CA>
Subject: CJD
To: "''" <>

Does anyone know of a decontamination procedure for benches or cryostats
after CJD exposure. Also, is there anyway to treat tissues with CJD?
We know of the phenol/formic acid method.
Are there other effective methods???????

Thanks in advance,

Gino Celebre, MLT
Department of Pathology and Molecular Medicine
Hamilton Health Sciences Corporation
Hamilton, Ontario, Canada
(905)527-4322 ext. 6145

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