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|From:||Tim Morken <firstname.lastname@example.org> (by way of histonet)|
Here is a lot of info, including good web sites, about CJD precautions
and disinfection. The CAP web site has good disinfection procedures.
Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
1600 Clifton Rd.
Atlanta, GA 30333
Handling Creutzveldt-Jakcob disease Tissues in the Histology Laboratory,
Titford, M. and Bastian, F.O., J Histotech, V12, No.3 Sept 1989
If you have access to the internet you can go to these sites:
Search Altavista for "CJD" at http://www.altavista.digital.com
there are many articles concerning CJD and V-CJD from Britain.
CAP: Detailed information on handling CJD in the autopsy suite and the
lab, with references.
For all CDC information on CJD, go to to the main CDC web page and
search for "CJD" :
CJD Epidemiology, risk factors and decontamination:
Udatated info at (reproduced below)
AGENT: Transmissible Spongiform Encephalopathies (Creutzfeldt-Jakob,
and related agents)
Laboratory-associated infections with the transmissible spongiform
diseases) have not been documented. However, there is evidence that
disease (CJD) has been transmitted iatrogenically to patients by
corneal transplants, dura
mater grafts and growth hormone extracted from human pituitary
glands, and by exposure
to contaminated electroencephalographic electrodes (99). Infection
is always fatal. There is
no known nonhuman reservoir for CJD or kuru. Nonhuman primates and
animals have been infected by inoculation, but there is no evidence
transmission. Scrapie of sheep and goats, bovine spongiform
encephalopathy and mink
encephalopathy are transmissible spongiform encephalopathies of
animals that are similar to
the human transmissible diseases. However, there is no evidence
that the animal diseases
can be transmitted to man.
LABORATORY HAZARDS: High titers of a transmissible agent have been
the brain and spinal cord of persons with kuru. In persons with
and its Gerstmann-Straussler-Schenker Syndrome variants, a similar
transmissible agent has
been demonstrated in the brain, spleen, liver, lymph nodes, lungs,
spinal cord, kidneys,
cornea and lens, and in spinal fluid and blood. Accidental
parenteral inoculation, especially
of nerve tissues, including formalin-fixed specimens, is extremely
non-nerve tissues are less often infectious, all tissues of humans
and animals infected with
these agents should be considered potentially hazardous. The risk
of infection from aerosols,
droplets, and exposure to intact skin, gastric and mucous membranes
is not known;
however, there is no evidence of contact or aerosol transmission.
These agents are
characterized by extreme resistance to conventional inactivation
irradiation, boiling, dry heat and chemicals (formalin,
however, they are inactivated by 1 N NaOH, sodium hypochlorite (>2%
concentration) and steam autoclaving at 134 degrees C for 1 hour.
RECOMMENDED PRECAUTIONS: Biosafety Level 2 practices and facilities
for all activities utilizing known or potentially infectious
tissues and fluids from
naturally-infected humans and from experimentally infected animals.
Extreme care must be
taken to avoid accidental autoinoculation, or other traumatic
parenteral inoculations of
infectious tissues and fluids (76). Although there is no evidence
to suggest that aerosol
transmission occurs in the natural disease, it is prudent to avoid
the generation of aerosols
or droplets during the manipulation of tissues or fluids, and
during the necropsy of
experimental animals. It is further strongly recommended that
gloves be worn for activities
that provide the opportunity for skin contact with infectious
tissues and fluids.
Formaldehyde-fixed and paraffin-embedded tissues, especially of the
infectious. It is recommended that formalin-fixed tissues from
suspected cases of
transmissible encephalopathy be immersed in 96% formic acid for 30
histopathologic processing (15). Vaccines are not available for use
in humans (51).
----Original Message Follows----
Date: Wed, 03 Mar 1999 09:24:22 -0500
From: "Celebre, Gino" <celebre@HAMCIVHOS.ON.CA>
To: "'email@example.com'" <firstname.lastname@example.org>
Does anyone know of a decontamination procedure for benches or cryostats
after CJD exposure. Also, is there anyway to treat tissues with CJD?
We know of the phenol/formic acid method.
Are there other effective methods???????
Thanks in advance,
Gino Celebre, MLT
Department of Pathology and Molecular Medicine
Hamilton Health Sciences Corporation
Hamilton, Ontario, Canada
(905)527-4322 ext. 6145
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