| From: | RUSS ALLISON <Allison@cardiff.ac.uk> |
| To: | tjohnso@nimr.mrc.ac.uk (Tammaryn Johnson) |
| Reply-To: | |
| Date: | Mon, 08 Mar 1999 10:59:08 +0000 (GMT0BST) |
| Content-Type: | text/plain; charset=US-ASCII |
Be very careful assessing the TUNEL reaction for apoptotic cells. Endogenous nucleases may give false positive results. This is a high risk in liver and gut. It (nuclease) may be inhibited by diethyl pyrocarbonate. Russ Allison, Wales