RE: troublesome tissues

<< Previous Message | Next Message >>
From:"Bartlett, Jeanine" <>
To:"'Tami Lee'" <>,
Date:Mon, 08 Mar 1999 10:12:17 -0500
Content-Type:text/plain; charset="iso-8859-1"

Tami Lee:

I have found that one of the most important steps in quality fixation is the
tissue size.  Make sure that when the pathologist is grossing the tissue
he/she does so as thinly as possible to allow for adequate fixation,
dehydration, and clearing.  I have also found that using Pen-Fix or a
fixative like Pen-fix really helped with fatty tissues such as breast
tissue.  Again, the thinner the better!

Good luck!

Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
1600 Clifton Rd., NE  MS/G-32
Atlanta, GA  30333

-----Original Message-----
From: Tami Lee []
Sent: Monday, March 08, 1999 8:51 AM
Subject: troublesome tissues

Hi everyone,
	I am trying to fix, process, and section breast and uterine tissue.

Although the uterus was fixed
in 10% NBF for several days, it still appeared to have areas that were
fixed.  The processing
schedules that I have been using are 12 hours long, and do not seem to be 
effective, especially
on the breast tissue.  Does anyone have any advice they wouldn't mind
I have not been able
to find anything in our laboratory's textbooks.
Thanks in advance,
Tami Lee

<< Previous Message | Next Message >>