Date:          Wed, 10 Mar 1999 15:55:40 -0500
From:          "Greer, Patricia" <pwg1@cdc.gov>
Subject:       RE: sticky slides
To:            "'jm36@st-andrews.ac.uk'" <jm36@st-andrews.ac.uk>,
               histonet@pathology.swmed.edu

Merck/BDH sell aproduct called Cedukol Cat# 4363 which is a Low 
Viscocity nitrocellose, ie celloidin.

Bill
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
 Jill,

>From many years ago, I remember that someone in our lab used a celloidin
covering over the sections which prevented their washing off under harsh
treatment such as with GMS staining.
The procedure is in the Manual of Histologic Staining Methods of the Armed
Forces Institute of Pathology (better known as the AFIF Manual), third
edition, 1960, page 29; and I quote:
"Paraffin sections which tend to become separated from the slide because of
the action of acids and alkalis used in the various staining procedures, and
which are extremely dried or which contain bone and keratinized material may
be held more firmly to the slide if they are couted with a thin film of 0.5
- 1% celloidin before staining.  
	1.  Deparaffinize the slide in xylene and run through several
changes of absolute alcohol.
	2.  Immerse in 0.5 - 1% celloidin solution for 1 to 2 minutes.
	3.  Air-dry sections for 30 minutes or until sections turn white.
	4.  Immerse sections in 80% alcohol for 1 to 2 minutes.
	5.  Rinse in distilled water and proceed with desired stain.
H.C. Cook conducted a study on section adhesives and their effects with
various treatments.  This study may be found in Stain Technology 40:321-328,
1965"

Celloidin may be hard to find but I think an electron microscopist may be a
good source to try.
I remember also that one of our resourceful techs tried spraying sections
with unscented (aerosol) hair spray (a cheap brand) as an alternative to
celloidin and it worked as well.

Hope this gives you some ideas.

Pat Greer

Patricia W. Greer
Infectious Disease Pathology
Centers for Disease Control
pwg1@cdc.gov

 
-----Original Message-----
From: jm36@st-andrews.ac.uk [mailto:jm36@st-andrews.ac.uk]
Sent: Tuesday, March 09, 1999 10:38 AM
To: histonet@pathology.swmed.edu
Subject: sticky slides


Does any one know of any super duper  sticky slides I can get hold of . I,ve
tried polysine,gold plus, old fashioned subbed etc ut my frozen tadpoles
keep falling  off. The procedures I am doing are frozen sections for an
enzyme and then potassium permanganate/oxalic bleach.


JILL.



Bill Sinai
Department Manager
Tissue Pathology
ICPMR Westmead Hospital 
WESTMEAD NSW AUSTRALIA
Phone 61+2+9845 7774  Fax 61+2+9687 2330