Dear Arlene,

Protocol here is as follows:

Fixation in alcohol-formalin, 1 minute
DI water rinse
Harris Hematoxylin, 30 sec,
Tap water rinse until clear
Bluing reagent, 10 dips
Tap water rinse, 10 dips
80% ETOH
Eosin, 6-10 dips, depending on how you like it.
95% ETOH
100% ETOH x2
Clear in xylene
Coverslip



Diana Goodwin, HT
Trenton,  NJ



> ----------
> From: 	Arlene N Taylor[SMTP:cloud39@juno.com]
> Sent: 	Friday, February 26, 1999 6:49 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	hematoxylin & eosin  frozen section staining
>
> Hi,
> I'm collecting  procedures for hematoxylin and eosin  of staining
> frozen
> sections.
> Thanks in advance.
>
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