Hi Arlene,
	The method we use is -
Fix in Wolman's solution ( 5% acetic in ethanol )   10 secs in microwave
( on high )
Hematoxylin ( Harris's ) 		30 secs
Blue in sat. lithium carbonate
Eosin  ( 1% in 1% calcium chloride )	45 secs
Note:	The microwave is an 800W domestic microwave ( high = full power
)
		Hope this helps,
			Alex Brown
			Crosshouse Hospital
			Kilmarnock, Scotland.
 ----------
From: Arlene N Taylor
To: histonet@pathology.swmed.edu
Subject: hematoxylin & eosin  frozen section staining
Date: 26 February 1999 23:49

Hi,
I'm collecting  procedures for hematoxylin and eosin  of staining
frozen
sections.
Thanks in advance.

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