RE:Smooth Muscle

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From:"Rogahn, Christina [PRI]" <>
To:"''" <>
Date:Fri, 12 Mar 1999 10:37:09 -0500

We routinely use SMA (Dako M0851) at 1:1000 (30 min RT) on both human and
rat tissue - beautiful signal, very clean.
For mice we use Dako's SMA-EPOS (U7033) (1 hr RT) anywhere from neat to 1:12
depending on the tissue.  SMA-EPOS is HRP conjugated - right into Dab after
the primary!  Another benefit is that we don't need to do any additional
blocking steps (except H2O2).  Hope this helps.
Chris Rogahn  

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