My experience is as Bryan's with Sirrus Red, I do not have Congo Red
experience, but we do use Sirrus Red with polarisers to see the cross linked
bundles of collagen in our bone tissue samples, and do see green
birefringence.
Patsy Ruegg

-----Original Message-----
From: Bryan Llewellyn
To: angelrj@email.uc.edu; histonet@Pathology.swmed.edu
Sent: 3/12/99 8:23 PM
Subject: Re: Sirius Red

My experience is that sirius red F3B does give green birefringence with
polarisers.  However, the colour is noticeably darker than the green
with
congo red.

Bryan Llewellyn

-----Original Message-----
From: angelrj@email.uc.edu <angelrj@email.uc.edu>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: March 12, 1999 11:40 AM
Subject: Sirius Red


>We have had several requests lately for Sirius Red.  We normally use
the
>Congo Red technique.  My question is:  Is there supposed to be the
green
>birefringence staining with the Sirius Red?  I've checked all my
>resources.  It is not mentioned in the few procedures I found and one
>book was contradictory.  I assume it is not supposed to since the
>sections are cut thinner (at 4-6 m.), and the majority of the
procedures
>didn't mention it.  I just want to ask others who are more experienced
>to make sure I have the facts.
>
>We do have a polarizer and there wasn't any birefringence even though
>the control had dark pink areas.
>
>Thanks for your help.
>
>Rita
>
>