RE: Microm Microtome
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From: | "Brody,Juanita X" <Juanita.X.Brody@kp.org> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
We switched from plastics using a JB4 microtome for various reasons one
being sensitivity to the reagents. Our technicians are able to get thin
sections using a Shandon 820-2 microtome one of our oldest. One of our
techs is suffering from repetitive motion disorder and unable to use a
rotary microtome. The pathologist rejects the sections from the automated
microtome. We recut them on the 820 and he is pleased. The manual
states the microtome will cut paraffin sections from .5 to 100 microns.
-----Original Message-----
From: emaher@zeiss.com [SMTP:emaher@zeiss.com]
Sent: Tuesday, March 02, 1999 7:34 PM
To: 'Histonet'; Brody;Juanita X
Subject: Re: Microm Microtome
There is no way you will get 1 micron sections off of a
paraffin
block. That's why people use plastic embedding for thin
sections.
The microtome will advance 1 micron but the paraffin is too
soft to
hold up to this small advance. It is also just about
impossible to
cut 1 micron frozen sections and they are much harder. If you
want to
cut at that setting, you need to embed in plastic, and this
microtome
will perform well.
______________________________ Reply Separator
_________________________________
Subject: Microm Microtome
Author: "Brody;Juanita X" <Juanita.X.Brody@kp.org> at Internet
Date: 2/26/99 2:34 PM
We purchased a motorized microtome, Zeiss HM355, to cut 1 micron
thick
paraffin sections of renal biopsies. We use low profile blades and
have
been unable to cut thin sections as required by the pathologist.
Does any
one have experience cutting thin sections on this microtome?
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