RE: Microm Microtome

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From:"Brody,Juanita X" <> (by way of histonet)
To:histonet <>
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We switched from plastics using a JB4 microtome for various reasons one
being sensitivity to the reagents.   Our technicians are able to get thin
sections using a Shandon 820-2 microtome  one of our oldest.  One of our
techs is suffering from repetitive motion disorder and unable to use a
rotary microtome.   The pathologist rejects  the sections from the automated
microtome.   We recut them on the 820 and he is pleased.    The manual
states the microtome will cut paraffin sections from .5 to 100 microns.

	-----Original Message-----
	From: []
	Sent:	Tuesday, March 02, 1999 7:34 PM
	To:	'Histonet'; Brody;Juanita X
	Subject:	Re: Microm Microtome

	     There is no way you will get 1 micron sections off of a
	     block.  That's why people use plastic embedding for thin
	     The microtome will advance 1 micron but the paraffin is too
soft to
	     hold up to this small advance.  It is also just about
impossible to
	     cut 1 micron frozen sections and they are much harder.  If you
want to
	     cut at that setting, you need to embed in plastic, and this
	     will perform well.

	______________________________ Reply Separator
	Subject: Microm Microtome
	Author:  "Brody;Juanita X" <> at Internet
	Date:    2/26/99 2:34 PM

	We purchased a motorized microtome, Zeiss HM355, to cut 1 micron
	paraffin sections of renal biopsies.   We use low profile blades and
	been unable to cut thin sections as required by the pathologist.
Does any
	one have experience cutting thin sections on this microtome?

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