Kathy,

There is an online procedure and reference that may be useful :

http://www.ccc.nottingham.ac.uk/~mpzjlowe/protocols/gallyas.html
<http://www.ccc.nottingham.ac.uk/~mpzjlowe/protocols/gallyas.html> 
© Copyright 1997 University of Nottingham Medical School Division of
Histopathology.

The method above stains tangles and nerve cell processes containing the
microtubule binding-protein tau,
found in Alzheimer's disease, but doesn't stain amyloid. It is said to be
more sensitive than the Modified Bielschowsky
stain for plaques and tangles (Yamamoto & Hirano, 1986)

Tangles can also be detected by IHC staining for phosphorylated tau protein.

                                      
Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center



	----------
	From:  Kathleen Pelton-Henrion [SMTP:PELTONK@mailbox.hscsyr.edu]
	Sent:  Wednesday, March 10, 1999 11:39 AM
	To:  HISTONET@PATHOLOGY.SWMED.EDU
	Subject:  Gallyas stain

	Could someone please suggest a protocol for the Gallyas Stain for
tangles
	and any helpful hints in doing the stain? Also, does this stain
stain the
	tangles the same way that the Biels does?
	Thanks in advance!
	Kathy