RE: Frozen Tissue

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From:"Garcia, Vicki {USA~Palo Alto}" <>
To:"'HistoNet Server'" <>
Date:Thu, 11 Mar 1999 15:23:53 -0800
Content-Type:text/plain; CHARSET=US-ASCII

Hi Harold-

The holes in the tissue probably result from your isopentane not being cold
enough.  We freeze muscle in isopentane cooled by liquid nitrogen.  The
isopentane is cold when it begins to turn viscous and the bottom of the
container turns white.  Also, if your isopentane gets too cold (begins to
freeze solid) it can slow freezing and you will end up with holes when you
You will have cracks in your tissue if you over freeze it.  Keep the tissue
submerged in the isopentane just long enough for it to freeze.
Hope this helps!  

Vicki Garcia
Roche Bioscience
Palo Alto, Ca.

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