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From:Alex Brown <>
To:"'Histonet'" <>
Date:Tue, 09 Mar 1999 13:08:00 +0000

Hi Gino,
	I don't have any up to the minute information, but I do have the
Advisory Committee on Dangerous Pathogens ( ACDP ) report on '
Precautions for work with human and animal Transmissible Spongiform
Encephalopies'  ( UK  1994 ). Their recommendations for disinfecting non
autoclavable ( if that's a word ) surfaces is to use sodium hypochlorite
( 20,000 ppm ) for one hour. However as this is fairly corrosive for
metals and some surfaces, 2M sodium hydroxide can be used as an
alternative. For the very few times we have had a CJD case we used the
sodium hydroxide method.  This report also states that phenol is
ineffective in decontaminating formalin treated tissue and should not be
used. The recommendation is to treat formalin fixed tissues with 96%
formic acid for one hour.
	The publication is available from the HMSO offices in London, so
if you need the address let me know. The guidelines may be different in
Canada of course, and there may well be more up to date information
		Hope this helps,
			Alex Brown
			Crosshouse Hospital
			Kilmarnock, Scotland.
From: Celebre, Gino
To: ''
Subject: CJD
Date: 03 March 1999 14:24

Does anyone know of a decontamination procedure for benches or cryostats
after CJD exposure. Also, is there anyway to treat tissues with CJD?
We know of the phenol/formic acid method.
Are there other effective methods???????

Thanks in advance,

Gino Celebre, MLT
Department of Pathology and Molecular Medicine
Hamilton Health Sciences Corporation
Hamilton, Ontario, Canada
(905)527-4322 ext. 6145

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