RE: Adenovirus

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"'histonet@pathology.swmed.edu'" <histonet@Pathology.swmed.edu>, "'Cathy Fragiskatos'" <FRAGISKA@PATHOLOGY.Lan.McGill.CA>
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Date:Fri, 12 Mar 1999 14:56:48 -0500
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Cathy,

We routinely run adenovirus on formalin-fixed paraffin sections.
Seralab cat# MAS173 mouse monoclonal(clone H-60) at 1:10,000 for 1 hour
at R.T. following HIER in citrate buffer pH6.0 10 min at 96C. detection
is with LSAB. Works like a charm!

Bryan

>----------
>From: 	Cathy Fragiskatos[SMTP:FRAGISKA@PATHOLOGY.Lan.McGill.CA]
>Sent: 	March 12, 1999 9:01 AM
>To: 	histonet@pathology.swmed.edu
>Subject: 	Adenovirus
>
>Dear histonetters,
>
>    Does anyone have any experience with adenovirus testing on 
>paraffin-embedded tissue? What is the most current method available to 
>detect adenovirus infection in tissue?
>
>With many thanks,
>
>Cathy Fragiskatos
>Royal Victoria Hospital
>Montreal, Quebec
>Canada.
>
>



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