RE: Acetylcholinesterases

<< Previous Message | Next Message >>
From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:Histonet@Pathology.swmed.edu, "'Sue.Hacker'" <Sue.Hacker@bbsrc.ac.uk>
Reply-To:
Date:Fri, 12 Mar 1999 09:41:46 -0500
Content-Type:text/plain

Sue,

There are two newer methods for the demonstration of true
acetylcholinesterase using a thiocholine substrate on cryostat sections. 
A permanent mount technique using formol-calcium-gum sucrose treated
sections (Filipe & Lake, 1983), and a glycerin mounted quantitative method
by Kugler ,1987.

I am not familiar with any commercially available kit. I do have both of the
above mentioned methods and would be glad to share them with you.

Eric Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center


	----------
	From:  Sue.Hacker [SMTP:Sue.Hacker@bbsrc.ac.uk]
	Sent:  Friday, March 12, 1999 6:44 AM
	To:  Histonet@Pathology.swmed.edu
	Subject:  Acetylcholinesterases

	Dear All,
	Has anyone any experience in acetylcholinesterases. we are looking
to 
	demonstrate Langerhans cells in bovine tissue using the above
technique.
	We are looking for an up to date method, Bancroft mentions the
Gerebtzoff
	method 1959. We wondered if there was a commercial kit available.
	Any help you can give would be appreciated.
	thanks,
	Sue Hacker,
	IAH Compton,
	Berks.



<< Previous Message | Next Message >>