MIB1 + bone marrow

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From:Heike Grabsch <h.grabsch@uni-koeln.de> (by way of histonet)
To:histonet <histonet@magicnet.net>
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anybody out there doing immunohistochemistry on bone marrow sections with
MIB 1? We get no staining so far. However, I have to admit that in
Germany the routine fixation for bone marrow biopsies contains a
combination of glutaraldehyde, formaldehyde and other ingredients. So, I
currently assume it is the glutaraldehyde which destroys the MIB1
But may be, there are some suggestions aout there? some special
pre-treatment? We use a pressure cooker (5 min) in citrate buffer at the
moment and a straeptavidin-biotin-hrp- detection system.

thanks for your help

Heike Grabsch, Germany

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