IHC Question

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From:Patsy.Ruegg@UCHSC.edu
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Wed, 10 Mar 1999 12:55:29 -0700
Content-Type:text/plain; charset="iso-8859-1"

I have been having trouble with non-specific background staining and have
gone to putting acetylated BSA in my wash buffer, which helps.  My question
is:  is there any reason not to also put the TWEEN in the wash buffer, it
seems that I read something a while back suggesting that they should not be
used together??????
Patsy Ruegg



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