Frozen Tissue

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From:Harold Obiakor <>
To:"'HistoNet Server'" <>
Date:Thu, 11 Mar 1999 17:46:26 -0500

Does anyone Know the best method to freeze tissue without having holes or
cracks in them? Currently we freeze our tissues with OCT (embedding medium
for frozen tissue) in Isopentane/Liquid nitrogen or dry ice and occasionally
we have gaping holes or cracks in the tissue after sectioning. We are
convinced that these holes are not due to the process of sectioning, but due
to the freezing method. We have also noticed that freezing tissues with OCT
in Isopentane on dry ice was a little bit better than in Isopentane placed
in liquid nitrogen.

								NIAID, NIH.

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