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From:Ms Louise Taylor <179LOU@chiron.wits.ac.za>
Date:Fri, 12 Mar 1999 10:19:26 +0000 (GMT)

Hi Histonetters

I have 2 queries
Firstly, has anyone made up the biotinyl Tyramide (home made recipe) 
for amplification of IHC. I have followed the recipe to detail 
Ref;J Path, vol.183:237-241(1997). As this makes up quite a bit of 
solution, I need to know if the biotinyl Tyramide will keep at 4'C or 
does one have to freeze it. what does one do with the stock solution 
? Keep it in the fridge/freezer? How stable is this solution?
The kit from Dako is rather expensive, so go figure!!
If anyone has a better protocol, please be so kind as to share it 
wiff me. Thanks.

Secondly, I am looking for an antibody called ALK-1 :Anaplastic 
Lymphoma Kinase-1. I have looked in the Linscott dictionary and 
nothing doing. Any info is welcome.

Zenobia Haffajee
SAIMR - Anatomical Pathology
South Africa

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