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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk> (by way of histonet)
To:histonet <histonet@magicnet.net>
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>Date: Thu, 04 Mar 1999 14:57:56 -0500
>From: "Patterson, Noelle" <PattersonN@nmripo.nmri.nnmc.navy.mil>
>To: Histonet <HistoNet@pathology.swmed.edu>
>MIME-version: 1.0
>
>Hi!  I am collecting cryostat section storage methods.  Please include
>whether you fix sections before storage and what temp. you store slides at.
>Do you use a dessicant or not?  Once stored, how do you prepare them for
>use/stainings.  Does your storage method permit later routine, special,
>and/or immunohistochemical stains with the same quality as same day cut and
>stained (not stored) specimens?
>
>Thanks,
>Noelle Patterson
>Naval Medical Research Center
>Bethesda, Md
>pattersonn@NMRIPO.NMRI.NNMC.NAVY.MIL
>

Noelle,
	Have only ever stored for histochemistry. Store at -80 in a sealed
box with some silca gel. Remove sections as necessary, bring back to
ambient and stain. Although ATPase works I find that it is better on fresh
sections. Human material is more forgiving in this respect than other
species.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk




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