Why do you need to get rid of GFP fluorescence if you are going to do IHC?
IHC is based on using chromogen with enzyme immunohistochemistry and if you
are not looking at your IHC protocol with a fluorescent microscope, but
rather with light microscopy, then GFP will not be seen - that would be
more of a problem with doing immunofluorescent staining (IFA).
If you mean doing another immunofluorscent stain for different tissue
component, or cell, then use a different color fluorophore i.e. red than
GFP, then you should not have GFP showing up, unless you switch to a GFP
filter (you could have double fluorescence then).
Maybe I am not understanding what you want to do here.
Gayle M. Callis
Bozeman MT 597175
----- Original Message -----
From: "Helen E Johnson"
Sent: Thursday, March 27, 2008 1:01 PM
Subject: [Histonet] quenching GFP fluorescence
> Hi Histonetters,
> How would you quench GFP fluorescence prior to IHC?
> Helen Johnson
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