I'm not sure that this matters for this, but DAB is not a substrate, but a 
chromogen.  The actual substrate is hydrogen peroxide.




MKing  
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/28/2008 10:21 AM

To
histonet@lists.utsouthwestern.edu
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Subject
[Histonet] quenching GFP fluorescence






Helen,
If you're using DAB as a peroxidase substrate for your IHC the GFP 
fluorescence will be eliminated.
Mike King
----------
Message: 9
Date: Thu, 27 Mar 2008 15:01:26 -0400
From: Helen E Johnson 
Subject: [Histonet] quenching GFP fluorescence
To: histonet@lists.utsouthwestern.edu
Message-ID:
 

 
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
       How would you quench GFP fluorescence prior to IHC?
                                     Helen Johnson
(hej01@health.state.ny.us)


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