Re: [Histonet] Sharpie Ink staining

From:Geoff McAuliffe

I noticed this years ago. I labeled a slide with Sharpie ink. The 
alcohol, or was it xylene, level in the Coplin jar was high enough to 
dissolve the ink. Voila, stained nuclei!
I guess I should have published it.


Bower, Jennifer wrote:
> I figured it out! All day I've been working on this idea, and it works!
> All you have to do is run your slides through to water, then take a
> Sharpie pen (I use black) and ink all over the sample, then put the
> slides through the rest of the protocol, just replacing the hematoxylin
> step with the application of the Sharpie. I got wonderful blue/black
> nuclei and crisp chromatin detail.
> -----Original Message-----
> From:
> [] On Behalf Of Joe
> Nocito
> Sent: Tuesday, April 01, 2008 5:24 PM
> To: Mark Tarango; Rittman, Barry R
> Cc:
> Subject: Re: [Histonet] Democracy
> ok, ok. Let's get to the real issues.
> If we don't have hematoxylin, can we use blueberry juice and vodka, or
> was 
> than gin?
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583

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