Re: [Histonet] SATB1 antibody

From:"Mark Tarango"

I've used this antibody looking at T-cells.  I used abcam ab49061 (1:50 -
1:100) for two hours with Diva Decloaker (biocare) 125 degrees C in the
pressure cooker and polymer detection (mach 4 from biocare).

I never stained breast with this protocol... only lymphoid tissue and bone
marrow.  Anyway, you can see the T-zone in the tonsil light up, and you'll
know it's working right.

On Fri, Apr 4, 2008 at 8:05 AM, Patti Loykasek 

> Hi all. TGIF. Quick question. Wondering if anyone is using an antibody to
> SATB1 on FFPE human tissues. If so, what antibody are you using? We are
> looking to use this antibody on breast cancer tissues. There are a couple
> of
> articles in the literature using this antibody, but unfortunately it is
> unclear in the articles exactly which antibody they are using. Thanks for
> the help.
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
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