I've used this antibody looking at T-cells. I used abcam ab49061 (1:50 -
1:100) for two hours with Diva Decloaker (biocare) 125 degrees C in the
pressure cooker and polymer detection (mach 4 from biocare).
I never stained breast with this protocol... only lymphoid tissue and bone
marrow. Anyway, you can see the T-zone in the tonsil light up, and you'll
know it's working right.
On Fri, Apr 4, 2008 at 8:05 AM, Patti Loykasek
> Hi all. TGIF. Quick question. Wondering if anyone is using an antibody to
> SATB1 on FFPE human tissues. If so, what antibody are you using? We are
> looking to use this antibody on breast cancer tissues. There are a couple
> articles in the literature using this antibody, but unfortunately it is
> unclear in the articles exactly which antibody they are using. Thanks for
> the help.
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
> This e-mail message, including any attachments, is for the sole use of the
> intended recipients and may contain privileged information. Any
> review, use, disclosure or distribution is prohibited. If you are not the
> recipient, please contact the sender by e-mail and destroy all copies of
> original message, or you may call PhenoPath Laboratories, Seattle, WA
> at (206) 374-9000.
> Histonet mailing list
Histonet mailing list