Re: [Histonet] Reprocessing tissue

From:"Mark Tarango"

You might want to scrape off any extra paraffin and make sure a cleaning
cycle has been run, but I think Joe has it right when he says that this is
the way to reprocess tissue.  Clean the bottles and the processor after the
run, but for the sake of the tissue, don't deparaffinize the tissue before

Keep in mind that he is melting any extra paraffin off before processing.
When he puts the cassettes in the container of formalin, any little bits of
paraffin probably float up to the top.  I'd think build-up in the machine
shouldn't be much of a problem.

On Mon, Mar 24, 2008 at 2:13 AM, Gudrun Lang  wrote:

> Joe,
> How many blocks did you reprocess in this manner at one time? I am
> concerned, if the processor suffers from too many paraffinized blocks in
> the
> retorte.
> When we were trained on our first VIP (1989) the technician stressed the
> importance of getting rid of the remaining paraffin in the retorte before
> starting a new run. He said, the danger is, that little hard particles of
> paraffin can get into the valves and block it.
> What do you think about this?
> Gudrun Lang
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
> -----Ursprüngliche Nachricht-----
> Von:
> [] Im Auftrag von Joe
> Nocito
> Gesendet: Montag, 24. März 2008 01:46
> An: Lynn Wade;
> Betreff: Re: [Histonet] Reprocessing tissue
> Lynn,
> ThermoFisher Scientific and Ventana Medical Systems have systems that you
> can barcode the paperwork, which then makes bar-coded blocks which then
> makes bar-coded slides. In each step, the barcodes are read to ensure that
> the correct specimen is being processed. If there is a mis-match, an alarm
> beeps alerting the user.
> For reprocessing tissue, we just melt the blocks down, place the tissue
> back
> in the block and put the blocks in formalin to be processed with new
> cases.
> Whatever area did not process the first time will take up the formalin,
> and
> graded alcohols. When the tissues reach xylene, the paraffin is dissolved
> and everything get infiltrated. The areas that have been processed will
> repel the formalin and alcohols until they are immersed into xylene. I
> find
> this method is a lot easier on the tissues, especially if IHC is performed
> on them later.
> As far a an electronically created list of blocks going into a processor,
> I
> haven't heard of any.
> Hope this helps.
> Joe
> ----- Original Message -----
> From: "Lynn Wade" 
> To: 
> Sent: Sunday, March 23, 2008 6:31 PM
> Subject: [Histonet] Reprocessing tissue
> Hi folks:
> I am wondering if anyone has had an incident in Patholgy lab where on the
> tissue processor the reagents got switched inadvertantly. For instance,
> the
> 80% alcohol was inadvertantly placed in the last 100% alcohol slot and
> thus
> water was reintroduced into the tissue just before xylene, clearing amd
> paraffin.
> Has anyone had this occur and how did you recover the tissue?
> Also, can anyone tell me if there is such a processor that has a system
> that
> can be used to log in the cassette numbers that are put onto the processor
> so that in the event of some incident such as we had the retrieval of the
> exact specimens can be done electronically?
> And lastly can anyone tell me if they have a fully barcoded system whereby
> path specimens arrive barcoded and every document, slide and block has a
> barcode that allows for tracking of the tissue at all times?
> We are looking at processes and trying to close some gaps.
> Lynn Wade
> Program Manager, Safety & Quality Management
> Medical Services & Diagnostics
> Eastern Health
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