I always used successfully the following procedure:
a- prepare a "diastase buffer": anhydrous sodium chloride (8 g) + anhydrous dibasic sodium phosphate (0.282 g) + anhydrous monobasic sodium phosphate (1.974 g) + distilled water UP TO 1 liter. This buffer will have a pH @ 6 and has to be kept in the refrigerator.
b- glycogen digesting solution: take 40 mL of the buffer and heat it to 37║C and add 0.5 g of diastase of malt (Fisher brand "maltin" cat.No. D22-100).
c- incubate the hydrated section at 37║C for 30 minutes
Proceed with the PAS, counter stain, etc., etc.
Josh Wray wrote:
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