Re: [Histonet] Immuno on decaled toenail????

From:"Joe Nocito"

This is a job for "Joe the Toe", hold on while I get my cape on.

OK, I'm back. Try soaking the block in 10% sodium hydroxide for about ten 
minutes, then put it back on ice.
May I suggest that you soak you toenails in 10% sodium hydroxide before 
processing? I leave my blocks in the solution for about an hour, followed by 
a water rinse before they go on the processor. We had a melanoma from a 
thumb on a 21 year old that we had to perform immunos on. First time I saw 
melanoma from a 21 year old and in a nail. The 10% NaOH did a really nice 
job and we were able to get really nice sections. Hope this helps.

----- Original Message ----- 
Sent: Friday, March 28, 2008 3:26 PM
Subject: [Histonet] Immuno on decaled toenail????

> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100 on a
> toenail?  Had any problems with staining after using a decal  solution?  I 
> have
> stained twice and pathologist is not happy with staining  pattern.  This 
> is
> the first nail that I've had to do a immunohistochemical  stain on to rule 
> out
> melanoma.  Any other advice on softening  toenail?  Sorry for all of the
> questions, but I know this is a great place  to start.  Thanks in advance.
> Sylinda Battle
> **************Create a Home Theater Like the Pros. Watch the video on AOL
> Home.
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