This is a job for "Joe the Toe", hold on while I get my cape on.
OK, I'm back. Try soaking the block in 10% sodium hydroxide for about ten
minutes, then put it back on ice.
May I suggest that you soak you toenails in 10% sodium hydroxide before
processing? I leave my blocks in the solution for about an hour, followed by
a water rinse before they go on the processor. We had a melanoma from a
thumb on a 21 year old that we had to perform immunos on. First time I saw
melanoma from a 21 year old and in a nail. The 10% NaOH did a really nice
job and we were able to get really nice sections. Hope this helps.
----- Original Message -----
Sent: Friday, March 28, 2008 3:26 PM
Subject: [Histonet] Immuno on decaled toenail????
> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100 on a
> toenail? Had any problems with staining after using a decal solution? I
> stained twice and pathologist is not happy with staining pattern. This
> the first nail that I've had to do a immunohistochemical stain on to rule
> melanoma. Any other advice on softening toenail? Sorry for all of the
> questions, but I know this is a great place to start. Thanks in advance.
> Sylinda Battle
> **************Create a Home Theater Like the Pros. Watch the video on AOL
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