Re: [Histonet] Immuno on decaled toenail????

From:Rene J Buesa

First toenails do not contain calcium and the keratin they are made does not respond adequately to acid hydrolysis. You will need an alkaline hydrolysis (10% NaOH for at least 30 minutes after fixation before processing).
  After that the IHC is likely to be weak and you will need some twinkling.
  René J. wrote:
  Hello Histoland,
Is there any techs that have performed an immuno stains such as S100 on a 
toenail? Had any problems with staining after using a decal solution? I have 
stained twice and pathologist is not happy with staining pattern. This is 
the first nail that I've had to do a immunohistochemical stain on to rule out 
melanoma. Any other advice on softening toenail? Sorry for all of the 
questions, but I know this is a great place to start. Thanks in advance.

Sylinda Battle

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