Toenails do not contain calcium, although an acid may perform some protein
hydrolysis to break down the protein to may help soften the keratin. This
may also damage the antigenic epitope. Some people use NAIR, or an alkaline
solution to soften the keratin, beware, high pH also damages alkaline
sensitive linkages too.
Histonet has many suggestions for softening toenails already, but mostly for
sectioning purposes. Check the archives and do a search.
Sounds like a difficult situation for this IHC.
Gayle M. Callis
Bozeman MT 59715
---- Original Message -----
Sent: Friday, March 28, 2008 2:26 PM
Subject: [Histonet] Immuno on decaled toenail????
> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100 on a
> toenail? Had any problems with staining after using a decal solution? I
> stained twice and pathologist is not happy with staining pattern. This
> the first nail that I've had to do a immunohistochemical stain on to rule
> melanoma. Any other advice on softening toenail? Sorry for all of the
> questions, but I know this is a great place to start. Thanks in advance.
> Sylinda Battle
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