Re: [Histonet] Hematoxylin Shortage??

From:"Joe Nocito"



I agree,
nothing like home made, that includes pies, cakes, lasagna. Sorry, went off 
on a tangent.

JTT
----- Original Message ----- 
From: "kemlo" 
To: "'Akemi Allison-Tacha'" ; "'Gayle Callis'" 
; "'Emily Sours'" ; 

Sent: Sunday, March 30, 2008 2:54 AM
Subject: RE: [Histonet] Hematoxylin Shortage??


> We used to have 5 litre flasks of haematoxylin ripening in the window for
> months; if you got taken short you had to artificially ripen using an
> oxidising agent. If you had enough stock then the sun did the work.
>
> I always thought "organic" haematoxylin stained better that the factory 
> made
> stuff but I do think it overoxidised at a slower rate and gave 'cleaner;
> results.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi
> Allison-Tacha
> Sent: 29 March 2008 19:55
> To: Gayle Callis; Emily Sours; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Hematoxylin Shortage??
>
> Hi All,
>
> Yes, Gayle's memory is still going strong!  I remember
> the boat rumor around 1976.  And yes, I too was making
> Harris hematoxylin with mercuric oxide "red".  We
> would let it ripen in the dark for a few weeks before
> using it.  What a wonderful sheen it had.  I remember
> a student was making it in the old Providence Hall
> nursing school before we moved to our new lab.  The
> student added the total amount of the mercuric oxide
> and it shot straight up to the ceiling and permanently
> stained it.   That was a heck of a lesson!
>
> Regards,
> Akemi
>
> --- Gayle Callis  wrote:
>
>> Not the goat dying, but more like a boat sinking.
>>
>> The last time there was a "rumored" hematoxylin
>> shortage, back in the 70's,
>> it was some boat sinking with all the Campechium
>> logs aboard.  I recall some
>> panic stockpiling of hematoxylin - the days when we
>> made the stain ourselves
>> with mercuric oxide.  I think the sunken boat rumor
>> was a figment of
>> someones vivid imagination.
>>
>> For all the hematoxylin historians out there,
>> correct me if I am wrong.
>>
>> Gayle Callis
>> HTL/HT/MT(ASCP)
>> Bozeman MT 59715
>>
>>
>> ----- Original Message ----- 
>> From: "Emily Sours" 
>> To: 
>> Sent: Friday, March 28, 2008 7:25 AM
>> Subject: Fwd: [Histonet] Hematoxylin Shortage??
>>
>>
>> > Why would this happen?
>> > Is this like that one goat dying at Santa Cruz?
>> >
>> > Emily
>> > -- 
>> > People aren't like chocolates. People are
>> bastards. Bastards with
>> > bastard coating and bastard filling.
>> >
>> > _______________________________________________
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>> >
>>
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>>
>>
>>
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>
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> Client Services Manager
> PhenoPath laboratories
> 551 North 34th Street, Suite 100
> Seattle, WA 98103-8675
> Work: (206) 374-9000
> Cell: (425) 941-4287
> E-Mail: akemiat3377@yahoo.com
>
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