Re: [Histonet] Choosing an antibody

From:"Gayle Callis"


This antibody is available from Serotec and spleen makes a good positive 
control.   There are photos available on websites to show the interesting 
pattern of these cells in the rat spleen.  We always detect with goat 
antimouse IgG isotype (whatever this is) -biotinylated, from Southern 
Biotechnology, and get no background.

Gayle M. Callis

----- Original Message ----- 
From: "Liz Chlipala" 
To: "Till, Renee" ; 
Sent: Monday, April 14, 2008 12:05 PM
Subject: RE: [Histonet] Choosing an antibody


F4/80 does not work on rat, it's a marker for activated macrophages in
mouse tisse.  For rat you want the clone ED-1.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504

-----Original Message-----
[] On Behalf Of Till,
Sent: Monday, April 14, 2008 11:55 AM
Subject: [Histonet] Choosing an antibody

I am searching for an F4/80 antibody to used on FFPE rat livers. I have
found two possibilities (though if anyone happens to have an antibody
suggestion please let me know) and I am wondering which would be better,
or if it makes much difference. They are both from the same company but
the sources differ.
One is a rabbit polyclonal raised against amino acids w/in and
extracellular domain, and the other is raised against a peptide mapping
at the C-terminus.
I have encountered this before and always wondered what is the
difference and if one is better. Thanks.

Renee' Till, HT (ASCP)

Research Assistant

Arkansas Children's Nutrition Center

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