Rene and Kim,
If you cover the waterbath (they come with covers) and also the slide
container (coplin jars, whatever), it should eliminate evaporation.
Waterbath methods are also found in the literature. Jules Elias has
published a method but I do not recall the temperature used overnight.
I have several waterbath heated methods that use higher temperatures for
shorter times if Kim is interested. I will be happy to provide those via
private message with attachment.
Gayle M. Callis
Bozeman MT 59715
----- Original Message -----
From: "Rene J Buesa"
To: "Kim O'Sullivan" ;
Sent: Saturday, April 12, 2008 9:03 AM
Subject: Re: [Histonet] Antigen retrieval
> Never heard of such a procedure. On the other hand, unless you are dealing
> with a HUGE amount of buffer, it will evaporate during such a prolonged
> period of time.
> Check your reference, I think something is not right.
> René J.
> Kim O'Sullivan wrote:
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